In the preparation of agarose gel, agarose powder will be...

In the preparation of agarose gel, agarose powder will be mixed with buffer. Agarose powders need to be weighed first before mixing it with buffer. When 1 gram agarose gel is added, it will be followed by 100 ml of buffer. The ratio is 1:100 for agarose powder and buffer respectively. Then, the solution of agarose powder and buffer is put in the microwave in order to melt the agarose until the solution become clear (Carson Robertson, 2005). Agarose gel is poured in the casting trays. Actually casting trays is available various in sizes and made of UV transparent plastic. Finally, the comb is used to make well in the gel where the dyed DNA will be loaded. According to Bio Rad (n.d.) comb is placed in slots on the casting tray. Usually,†¦show more content†¦Besides, EtBr also function to alter DNA mass, rigidity and mobility, so it is one of the best factors that affect the mobility of the DNA. EtBr need to be stored in the dark because they are sensitive to light. To mix DNA sample and loading buffer, usually Parafilm is used. The sample will be pipette on the mini-gel. After that, the tank lid is placed, the current will be applied. When stained with Ethidium bromide, the gel is viewed with an ultraviolet (UV) transilluminator (Environment, Health Safety 2010). Usually for standard transilluminator it will use the wavelength of 302/312-nm (UV-B). Then, the DNA fragment will migrate through the gel and illumin ation with the UV light will cause the intercalated dye to fluorescent with a pale pink colour. The exposure of the UV light should be limited because exposure over the time limit will cause the DNA to damage. Then, the transilluminator setting will change to the highest in order to photograph the mini-gel (Farrell, 2010). The larger fragment will fluoresce more intensely compare to small fragment because larger fragment has more mass of DNA so it will take up more dye. In this Agarose Gel Electrophoresis (AGE) Analysis, we run our entire DNA from the previous experiment 2A, 2B, and 2C; bacterial, plant and also animal’s DNA sample. Based on the Figure 1(focus on the red circle), the separation of DNA fragment for 7a, 7b, and 7c from the AGE diagram